Anti-NFH (Neurofilament Heavy Chain) Antibody

NFH protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion” (1980) . Biochem. J. 191 (2) : 543-546], followed by Prepcell purification (Bio-Rad) . Antibodies were prepared by injecting purified NFH protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. These antibodies have been found to cross-react with NFH protein from a variety of species, including human, mouse and rat.

Product Specifications

Background

Human Neurofilament Heavy Chain (NFH) is a 115,378 dalton protein (1072 amino acids) that forms part of the neuronal cytoskeleton. NFH is physically associated with 10 nm intermediate filaments and is said to be one of the "neurofilament triplet" proteins, although it probably serves more to stabilize the cytoskeleton rather than as an integral structural component. NFH immunoreactivity is found in neuronal somata, dendrites and axons.

Synonyms

Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

NEFH KIAA0845 NFH

UniProt

P12036

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Reactivity

Human, Mouse, and Rat

Immunogen

Native NF-H purified from bovine spinal cord

Immunogen Type

Cow

Target

Neurofilament Heavy Chain (NFH)

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

IHC, ICC, WB

Field of Research

Cell Markers Neuronal Markers Axonal Markers Neurodegenerative Disease

Assay Type

Primary Antibody

Assay Protocol

Chickens were immunized with NF-H protein purified from adult bovine brains. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks.

Concentration

1 mg/mL

Format

IgY Fraction

Form

IgY Fraction

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative

Molecular Weight

200-220 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

IHC Dilution

1:2000-1:5000

WB Dilution

1:5000-1:10,000

Pre Treatment

Chickens were immunized with NF-H protein purified from adult bovine brains. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks.

Target Description

ICC Dilution

1:2000-1:5000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:50000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.