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Anti-Nestin Antibody

Three antipeptide antibodies were generated in chickens against a sequence in mouse (NP_057910) gene product. Antibodies were affinity-purified and mixed in equal concentration. These antibodies have been validated with human, mouse and rat tissues.

Product Specifications

Background

Mouse Nestin is a 206,994 dalton protein (1864 amino acids) expressed in neural crest cells, CNS neural stems cells, as well as a few non-neural cell types in the embryo, including cells within the pancreatic islets of Langerhans and the limb bud. Nestin is an intermediate filament-associated protein, and contributes to the cytoskeleton.

Synonyms

Nestin

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

NES Nbla00170

UniProt

Q6P5H2

Host

Chicken

Species Reactivity

Mouse , Rat

Reactivity

Mouse and Rat

Immunogen

Synthetic peptides

Immunogen Type

Mouse

Target

Nestin

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

IHC, ICC, WB

Field of Research

Cell Markers Neural Stem Cell Markers Glial and Schwann Cell Markers

Assay Type

Primary Antibody

Assay Protocol

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the mouse nestin gene product (NM_NP_057910), but are shared with the rat (NM_012987, NCBI) protein sequence. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Concentration

300 µg/mL

Format

Affinity-Purified IgY

Form

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

250 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

Product Datasheet

https://cdn.shopify.com/s/files/1/0512/5793/4009/files/NES_datasheet.pdf

IHC Dilution

1:1000-1:2000

WB Dilution

1:10000-1:20000

Pre Treatment

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the mouse nestin gene product (NM_NP_057910), but are shared with the rat (NM_012987, NCBI) protein sequence. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Target Description

Mouse Nestin is a 206,994 dalton protein (1864 amino acids) expressed in neural crest cells, CNS neural stems cells, as well as a few non-neural cell types in the embryo, including cells within the pancreatic islets of Langerhans and the limb bud. Nestin is an intermediate filament-associated protein, and contributes to the cytoskeleton.

ICC Dilution

1:1000-1:2000

Quality Control

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:20000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Brid

AB_2314882

Uniprot Accession Number

Q6P5H2

Physical Properties

Liquid

Isotype

IgY

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