Anti-mCherry Antibody

Our Anti-mCherry chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects mCherry, and is affinity purified. It is great for use in ICC, WB.

Product Specifications

Background

MCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004) . Fluorescent proteins are commonly used to tag proteins in order to localize their expression in both cultured cells and in vivo. Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired. Our chicken anti-mCherry antibody is a highly sensitive reagent for detection of mCherry by western blot, immunocytochemistry, and immunohistochemistry.

Short Description

Our Anti-mCherry chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects n/a mCherry, and is affinity purified. It is great for use in ICC, WB.

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

MCHERRY

UniProt

X5DSL3

Host

Chicken

Immunogen

Purified recombinant mCherry (Uniprot X5DSL3) expressed in E.Coli

Immunogen Type

Purified recombinant mCherry (Uniprot X5DSL3) expressed in E.Coli

Target

MCherry

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

ICC, WB

Field of Research

Anti-Tag Antibodies

Assay Type

Primary Antibody

Assay Protocol

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

Concentration

0.2 mg/mL

Format

Affinity purified

Form

Affinity purified

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

27 kDa for native monomer

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

WB Dilution

1:500-1:1000

Pre Treatment

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

Target Description

mCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004). Fluorescent proteins are commonly used to tag proteins in order to localize their expression in both cultured cells and in vivo. Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired. Our chicken anti-mCherry antibody is a highly sensitive reagent for detection of mCherry by western blot, immunocytochemistry, and immunohistochemistry.