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Anti-Myelin Basic Protein (MBP) Antibody

An antipeptide antibody was generated in chickens against a sequence shared between the mouse (NP_034907) and human (NP_002376) gene products. Antibodies were affinity-purified and the concentration adjusted to 100 µg/mL.

Product Specifications

Background

Human Myelin Basic Protein (MBP) is a 20,115 dalton protein (186 amino acids) found in myelin of the CNS and PNS. Expressed as an intracellular protein by oligodendrocytes and Schwann cells, MBP aids in the compaction and stability of myelin.

Synonyms

Myelin basic protein, MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

MBP

UniProt

P02686

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Reactivity

Human, Mouse, and Rat

Immunogen

Synthetic peptide

Immunogen Type

Human, Mouse, Rat

Target

Myelin Basic Protein (MBP)

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

IHC, ICC, WB

Field of Research

Cell Markers Glial and Schwann Cell Markers

Assay Type

Primary Antibody

Assay Protocol

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the MBP gene product shared between the human (NP_002376, NCBI) and mouse (NP_034907, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentrations of the eluates were then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Concentration

100 µg/mL

Format

Affinity-Purified IgY

Form

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

21, 18, 17, 14 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

IHC Dilution

1:1000-1:2000

WB Dilution

1:1000-1:2000

Pre Treatment

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the MBP gene product shared between the human (NP_002376, NCBI) and mouse (NP_034907, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentrations of the eluates were then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Target Description

Human Myelin Basic Protein (MBP) is a 20,115 dalton protein (186 amino acids) found in myelin of the CNS and PNS. Expressed as an intracellular protein by oligodendrocytes and Schwann cells, MBP aids in the compaction and stability of myelin.

ICC Dilution

1:1000-1:2000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Brid

AB_2313550

Uniprot Accession Number

P02686

Physical Properties

Liquid

Isotype

IgY

Documents

Product Datasheet

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