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Anti-Microtubule-Associated Protein (MAP2) Antibody

Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (P20357) and human (NP_002365) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

Product Specifications

Background

We produce our Anti-Microtubule-Associated Protein (MAP2) chicken polyclonal primary antibody. It is great in IHC, ICC, WB and is affinity-purified IgY.

Synonyms

Microtubule-associated protein 2, MAP-2

Gene Name

MAP2

UniProt

P11137

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Immunogen

Human, Mouse, Rat

Immunogen Type

Synthetic peptides

Target

Microtubule-Associated Protein (MAP2)

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

IHC, ICC, WB

Assay Type

Primary Antibody

Concentration

200 µg/mL

Format

Liquid

Form

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

280 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

Product Datasheet

https://cdn.gentaur.com/products/588/688a1e1a118489d09abbf682/datasheet/map.pdf

IHC Dilution

1:1000-1:2000

WB Dilution

1:5000-1:10000

Pre Treatment

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the MAP-2 gene product, but are shared between the human (NP_002365, NCBI) and mouse (P20357, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Target Description

Human MAP-2 is a 199,296 dalton protein (1827 amino acids) expressed in neurons of the PNS and CNS, where it serves as a major component of the neuronal cytoskeleton. MAP-2 contributes to structural integrity and cell shape.

ICC Dilution

1:1000-1:2000

Quality Control

Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Brid

AB_2313549

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