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Polyhistidine Anti-6XHIS (for Westerns) Antibody

Our anti-6XHIS antibody recognizes the 6X-HIS tag with high titre and high specificity in western blot applications.

Product Specifications

Background

The synthetic peptide 6X-Histidine (HHHHHH) .

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Host

Chicken

Immunogen

Synthetic peptide

Immunogen Type

Synthetic peptide

Target

6XHIS

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

WB

Assay Type

Primary Antibody

Assay Protocol

Chickens were immunized with the 6X-Histidine peptide conjugated to keyhole limpet hemocyanin (KLH) and emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using 6X-Histidine peptide conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.

Concentration

10 mg/mL

Format

Mixture of IgY fraction and affinity-purified antibodies

Form

Mixture of IgY fraction and affinity-purified antibodies

Buffer

Sodium phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS, " pH 7.2) (50% by volume) with glycerol (50% by volume) as an anti-freezing agent and sodium azide (0.02%, w/v) as a preservative

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Shelf Life

12 months from receipt of product

WB Dilution

1:5000-1:10000

Pre Treatment

Chickens were immunized with the 6X-Histidine peptide conjugated to keyhole limpet hemocyanin (KLH) and emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using 6X-Histidine peptide conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.

Target Description

The synthetic peptide 6X-Histidine (HHHHHH).

Quality Control

This antibody preparation was analyzed by western blotting (at a dilution of 1:10000) using tissue extracts expressing a recombinant protein tagged with the 6X-HIS markerSecondary antibodies were HRP-labeled affinity-purified goat anti-chicken IgY (1:5000 dilution, Aves Labs Cat.# H-1004) .

Brid

AB_2313518

Physical Properties

Liquid

Isotype

IgY

Documents

Product Datasheet

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