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Anti-Growth-Associated Protein-43 (GAP-43) Antibody

This antipeptide IgY antibody was generated in chickens against a C-terminal sequence shared between the mouse (NP_032109) and rat (NP_05889.1) gene products. It has been validated with human, mouse and rat tissues.

Product Specifications

Background

We produce our Anti-Growth-Associated Protein-43 chicken polyclonal primary antibody. It is great in IHC, ICC, WB and is IgY fraction.

Synonyms

Neuromodulin, Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, pp46)

Gene Name

GAP43

UniProt

P17677

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Immunogen

Mouse

Immunogen Type

Synthetic peptide

Target

Growth-Associated Protein-43

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

IHC, ICC, WB

Assay Type

Primary Antibody

Concentration

2 mg/mL

Format

Liquid

Form

IgY Fraction

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative

Molecular Weight

43 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

Product Datasheet

https://cdn.gentaur.com/products/588/688a1e1a118489d09abbf661/datasheet/gap43.pdf

IHC Dilution

1:500-1:1000

WB Dilution

1:1000-1:2000

Pre Treatment

Chickens were immunized with a synthetic peptide corresponding to the C-terminal region of mouse GAP-43 coupled to keyhole limpet hemocyanin. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/mL. This preparation was then diluted 1:10 with PBS containing bovine serum albumin as a carrier. Finally, the antibody preparation was filter-sterilized.

Target Description

Growth-Associated Protein-43 is a 274 amino acid cytoplasmic protein found in neurons and various other embryonic cell types. This protein also goes by a number of alternative names, including neuromodulin and F1. In cultured neurons, GAP-43 antibodies selectively stain the growth cones of axons. As the substrate for various phosphorylation events, it is believed that GAP-43 plays a critical role in axonal growth, although its exact function is still unclear.

ICC Dilution

1:500-1:1000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:1000) using fluorescein-labeled goat anti-chicken IgY (1:1000 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Brid

AB_2313546

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