Anti-C-MYC Epitope Tag Antibody

This product is an unlabeled, affinity-purified chicken anti-C-MYC epitope antibody.

Product Specifications

Background

The synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL) .

Synonyms

CMyc, c-Myc, Myc

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Host

Chicken

Immunogen

Synthetic peptide

Immunogen Type

Synthetic peptide

Target

C-MYC Epitope Tag Antibody

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

ELISA, IHC, ICC, IP, WB

Field of Research

Anti-Tag Antibodies

Assay Type

Primary Antibody

Assay Protocol

Chickens were immunized with a synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL) conjugated to keyhole limpet hemocyanin (KLH) . After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentration of the eluate was then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Concentration

100 µg/mL (based on absorbance at 280 nm)

Format

Affinity-Purified IgY

Form

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

IHC Dilution

1:100-1:250

WB Dilution

1:500-1:1000

Pre Treatment

Chickens were immunized with a synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL) conjugated to keyhole limpet hemocyanin (KLH). After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentration of the eluate was then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Target Description

The synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL).

ICC Dilution

1:100-1:250

Quality Control

Antibodies were analyzed using ELISA and western blots using an N-terminal FLAG®-tagged protein. Secondary antibodies used in these quality control assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies (Aves Labs), respectively.