Anti-Metabotropic Glutamate Receptor Type 2/Type 3 (mGluR2/mGluR3) Antibody

We have three different anti-mGluR antibodies, each of which binds to a specific peptide sequence (s) present in different sets of mGluR gene family members. The ER2/3 antibody represents sequences found in both mGluR2 and mGluR3. (Note that this product cannot distinguish between these two receptors.) It comprises two antipeptide antibodies generated against overlapping sequences specific to the mGluR2 and mGluR3 gene products but not found in other mGluR gene family members. This antibody has been validated for immunohistochemistry and immunocytochemistry using mouse brain sections and cultured neuroblastoma cells as well as in rat brain sections.

Product Specifications

Background

The metabotropic glutamate receptors (mGluRs) represent a gene family of G-protein-coupled receptors, all of which bind the amino acid glutamate (the major excitatory neurotransmitter of the nervous system) but differ in their primary amino acid structure, their pharmacology, and their distribution in the peripheral and central nervous systems. In general, mGluR1 and mGluR5 receptors are post-synaptic and mGluR2 and mGluR3 receptors are pre-synaptic.

Synonyms

Metabotropic glutamate receptor 2, mGluR2, Metabotropic glutamate receptor 3, mGluR3

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

GRM2 GPRC1B MGLUR2 (mGluR2) and GRM3 GPRC1C MGLUR3 (mGluR3)

UniProt

Q14416

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Reactivity

Human, Mouse, and Rat

Immunogen

Synthetic peptides

Immunogen Type

Human, Mouse, Rat

Target

Metabotropic Glutamate Receptor Type 2 / Type 3 (mGluR2 / mGluR3)

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

IHC, ICC

Field of Research

Neurotransmitter Receptors

Assay Type

Primary Antibody

Assay Protocol

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to unique sequences within the metabotropic glutamate receptor type 2 and type 3 gene products, but was shared between the human (Q14416.2, Q14832.2 NCBI) and mouse (Q14B12.2, Q9QYS2.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, the concentrations of the eluates adjusted to 200 µg/mL, and the preparation was filter-sterilized.

Concentration

200 µg/mL

Format

Affinity-Purified IgY

Form

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

99 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

IHC Dilution

1:1000-1:2000

Pre Treatment

Target Description

ICC Dilution

1:1000-1:2000

Quality Control

This anti-peptide antibody preparation was analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.