Anti-CNPase (Cyclic Nucleotide Phosphodiesterase) Antibody

This antipeptide antibody was generated in chickens against a sequence shared between the mouse (P16330) and human (NP_149124) gene products. The antibody was affinity-purified, and its concentration was adjusted to 100 µg/mL. This antibody has been validated with human, mouse and rat tissues.

Product Specifications

Background

Murine CNPase (EC 3.1.4.37) is a 47,122 dalton protein (420 amino acids) found in myelin. This protein is expressed by oligodendrocytes of the CNS and by Schwann cells of the PNS.

Synonyms

2',3'-cyclic-nucleotide 3'-phosphodiesterase, CNP, CNPase, EC 3.1.4.37

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

CNP

UniProt

P09543

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Reactivity

Human, Mouse, and Rat

Immunogen

Synthetic peptide

Immunogen Type

Human, Mouse, Rat

Target

Murine CNPase (EC 3.1.4.37)

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

IHC, ICC, WB

Field of Research

Cell Markers Glial and Schwann Cell MarkersOligodendrocyte MarkersSchwann Cell Markers

Assay Type

Primary Antibody

Assay Protocol

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the CNPase gene product shared between the human (NP_149124, NCBI) and mouse (P16330, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentrations of the eluates were then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Concentration

100 µg/mL

Format

Affinity-Purified IgY

Form

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

47 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

IHC Dilution

1:500-1:1000

WB Dilution

1:500-1:1000

Pre Treatment

Target Description

Murine CNPase (EC 3.1.4.37) is a 47,122 dalton protein (420 amino acids) found in myelin. This protein is expressed by oligodendrocytes of the CNS and by Schwann cells of the PNS.

ICC Dilution

1:500-1:1000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.