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Anti-Choline Acetyltransferase (ChAT) Antibody

This antipeptide antibody was generated in chickens against a sequence shared between the mouse (Q03059) and human (P28329) gene products. The antibody was affinity-purified, and its concentration was adjusted to 100 µg/mL. This antibody has been validated with human, mouse and rat tissues.

Product Specifications

Background

We produce our Anti-Murine choline-O-acetyltransferase (EC 2.3.1.6) chicken polyclonal primary antibody. It is great in IHC, ICC and is affinity-purified IgY.

Synonyms

Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, EC 2.3.1.6

Gene Name

CHAT

UniProt

P28329

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Immunogen

Human, Mouse, Rat

Immunogen Type

Synthetic peptide

Target

Murine choline-O-acetyltransferase (EC 2.3.1.6)

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

IHC, ICC

Assay Type

Primary Antibody

Concentration

100 µg/mL

Dilution

WB Brain : : Not Recommended

Format

Liquid

Form

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

72 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

Product Datasheet

https://cdn.gentaur.com/products/588/688a1e1a118489d09abbf68d/datasheet/cat.pdf

IHC Dilution

1:1000-1:2000

WB Dilution

Not Recommended

Pre Treatment

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the Choline Acetyltransferase gene product shared between the human (P28329, NCBI) and mouse (Q03059, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentrations of the eluates were then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Target Description

Murine choline-O-acetyltransferase (EC 2.3.1.6) is a 71,721 dalton protein (640 amino acids) expressed in cholinergic neurons of both the PNS and CNS. In the CNS, ChAT is expressed in motor neurons and pre-ganglionic autonomic neurons of the spinal cord, a subset of neurons in the neostriatum, and in the basal forebrain. In the PNS, ChAT is expressed in a small subpopulation of sympathetic neurons and in all parasympathetic neurons. ChAT is the enzyme responsible for synthesis of acetylcholine from acetyl-coenzyme A and choline.

ICC Dilution

1:1000-1:2000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Brid

AB_2313537

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