Anti-Actin (Nitrocellulose Membrane Loading Control) Antibody

Our anti-actin antibody (made against rabbit muscle actin) recognizes mouse, rat and human actin proteins in SDS-polyacrylamide gel transfers onto nitrocellulose membranes. This loading control assures that similar amounts of total proteins were added to each of the lanes in the gels. Note that this product does not work in transfers using PVDF membranes.

Product Specifications

Background

Purified actin (42 kDa) isolated from rabbit muscle

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

UniProt

P63267

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Reactivity

Human, Mouse, and Rat

Immunogen

Actin purified from Rabbit muscle

Immunogen Type

Rabbit

Target

Actin

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

Assay Type

Primary Antibody

Assay Protocol

Chickens were immunized with purified actin from rabbit muscle emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using actin conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.

Concentration

10 mg/mL

Dilution

WB Brain : 1:1,000-1:2,000

Format

Mixture of IgY fraction and affinity-purified antibodies

Form

Mixture of IgY fraction and affinity-purified antibodies

Buffer

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.

Molecular Weight

42 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Shelf Life

12 months from receipt of product

WB Dilution

1:1,000-1:2,000

Pre Treatment

Target Description

Purified actin (42 kDa) isolated from rabbit muscle

Quality Control

This anti-actin IgY preparation was analyzed by western blot analysis (at a dilution of 1:2000) using HRP-labeled goat anti-chicken IgY (1:5000 dilution, Aves Labs Cat.# H-1004) as the secondary reagent.