Anti-Actin (Nitrocellulose Membrane Loading Control) Antibody
Our anti-actin antibody (made against rabbit muscle actin) recognizes mouse, rat and human actin proteins in SDS-polyacrylamide gel transfers onto nitrocellulose membranes. This loading control assures that similar amounts of total proteins were added to each of the lanes in the gels. Note that this product does not work in transfers using PVDF membranes.
Product Specifications
Background
We produce our Anti-Actin chicken polyclonal primary antibody. It is great in WB and is mixture of IgY fraction and affinity-purified.
UniProt
P63267
Host
Chicken
Species Reactivity
Human, Mouse, Rat
Immunogen
Rabbit
Immunogen Type
Actin purified from Rabbit muscle
Target
Actin
Clonality
Polyclonal
Isotype
IgY
Conjugation
Unconjugated
Applications
WB
Assay Type
Primary Antibody
Concentration
10 mg/mL
Dilution
WB Brain : 1:1,000-1:2,000
Format
Liquid
Form
Mixture of IgY fraction and affinity-purified antibodies
Buffer
Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%,w/v) as an anti-microbial agent.
Molecular Weight
42 kDa
Precautions
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Shipping Conditions
Ice packs
Storage Conditions
Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.
Shelf Life
12 months from receipt of product
Product Datasheet
https://cdn.gentaur.com/products/588/688a1e1a118489d09abbf67e/datasheet/act-1040.pdf
WB Dilution
1:1,000-1:2,000
Pre Treatment
Target Description
Purified actin (42 kDa) isolated from rabbit muscle
Quality Control
Brid
AB_2313503
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