Anti-Beta-Amyloid Peptide (N-terminus) Antibody

Beta-Amyloid peptide is a 40- or 42-amino acid fragment of the human Beta-Amyloid Peptide: Precursor Protein (770 amino acids) produced by the proteolytic actions of Beta and Gamma-secretases. Both forms of Beta-Amyloid peptide are rather insoluble and tend to self-aggregate into distinctive extracellular "plaques." These plaques are evident in brains from patients with Alzheimer's disease as well as in brains from individuals with a history of traumatic head injuries. In the case of Alzheimer's disease, it has been suggested that these extracellular Beta-Amyloid peptide plaques are themselves cytotoxic (rather than simply being markers of brain pathology), and are responsible for the dendritic pruning and other neurodegenerative changes seen.

Amyloid-beta A4 protein (ABPP) (APPI) (APP) (Alzheimer disease amyloid protein) (Amyloid precursor protein) (Amyloid-beta precursor protein) (Cerebral vascular amyloid peptide) (CVAP) (PreA4) (Protease nexin-II) (PN-II) [Cleaved into: N-APP; Soluble APP-alpha (S-APP-alpha) ; Soluble APP-beta (S-APP-beta) ; C99 (Beta-secretase C-terminal fragment) (Beta-CTF) ; Amyloid-beta protein 42 (Abeta42) (Beta-APP42) ; Amyloid-beta protein 40 (Abeta40) (Beta-APP40) ; C83 (Alpha-secretase C-terminal fragment) (Alpha-CTF) ; P3 (42) ; P3 (40) ; C80; Gamma-secretase C-terminal fragment 59 (Amyloid intracellular domain 59) (AICD-59) (AID (59) ) (Gamma-CTF (59) ) ; Gamma-secretase C-terminal fragment 57 (Amyloid intracellular domain 57) (AICD-57) (AID (57) ) (Gamma-CTF (57) ) ; Gamma-secretase C-terminal fragment 50 (Amyloid intracellular domain 50) (AICD-50) (AID (50) ) (Gamma-CTF (50) ) ; C31]

Synthetic peptide (DAE FRH DSG YEV HHQ KL) contained within both the 40- and 42 amino acid versions of the human Beta-Amyloid peptide (Amyloid Precursor Protein residues #672-688) .

Chickens were immunized with a synthetic peptide (corresponding to DAE FRH DSG YEV HHQ KL, residues #1-17 of the amyloid beta-peptide) coupled to keyhole limpet hemocyanin (KLH) . After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column to which the same peptide was attached. Finally, the antibody concentration was adjusted to 100 ug/ml and filter-sterilized.

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Chickens were immunized with a synthetic peptide (corresponding to DAE FRH DSG YEV HHQ KL, residues #1-17 of the amyloid beta-peptide) coupled to keyhole limpet hemocyanin (KLH). After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column to which the same peptide was attached. Finally, the antibody concentration was adjusted to 100 ug/ml and filter-sterilized.

Beta-Amyloid peptide is a 40- or 42-amino acid fragment of the human Beta-Amyloid Peptide: Precursor Protein (770 amino acids) produced by the proteolytic actions of Beta and Gamma-secretases. Both forms of Beta-Amyloid peptide are rather insoluble and tend to self-aggregate into distinctive extracellular "plaques." These plaques are evident in brains from patients with Alzheimer's disease as well as in brains from individuals with a history of traumatic head injuries. In the case of Alzheimer's disease, it has been suggested that these extracellular Beta-Amyloid peptide plaques are themselves cytotoxic (rather than simply being markers of brain pathology), and are responsible for the dendritic pruning and other neurodegenerative changes seen.

Antibodies were analyzed by immunohistochemistry (at a concentration of 3 µg/mL) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) or HRP-labeled goat anti-chicken IgY (1:2000 dilution, Aves Labs, Cat.# H-1004) as the secondary reagent.