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CTCF (isoform 1) Antibody

Goat polyclonal antibody to CTCF

Product Specifications

Product Name Alternative

CTCF; CCCTC-binding factor (zinc finger protein) ; 11 zinc finger transcriptional repressor; 11-zinc finger protein; CTCFL paralog; transcriptional repressor CTCF

Reactivity

Human

Target

CTCF (isoform 1)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

KKSKLRYTEEGKD

Field of Research

Epigenetics & Chromatin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:4000, WB: 0.03-0.3 μg/ml

Molecular Weight

82.8; 46.0

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 150kDa band observed in lysates of cell line Jurkat and nuclear Jurkat (calculated MW of 82.8kDa according to NP_006556.1) . The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

10664

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