Anti-MyD88 (IN) Antibody

Rabbit Polyclonal Antibody specific to MYD88 (IN)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-MYD88 (IN) Rabbit Polyclonal Antibody

Gene Name

MYD88

Gene ID

4615

NCBI Gene ID

UniProt

Q99836

Accession Number

NP_002459

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Peptide corresponding to aa 233-248 of human MyD88. The sequence differs from mouse MyD88 by two amino acids.

Target Antigen

Myeloid differentiation primary response protein MyD88

Target

MyD88 (IN)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IHC-P, IF, IP

Field of Research

Neuroscience

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed:15361868, PubMed:18292575, PubMed:33718825). Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:15361868, PubMed:24316379, PubMed:19506249). Increases IL-8 transcription (PubMed:9013863). Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Upon TLR8 activation by GU-rich single-stranded RNA (GU-rich RNA) derived from viruses such as SARS-CoV-2, SARS-CoV and HIV-1, induces IL1B release through NLRP3 inflammasome activation (PubMed:33718825). MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (By similarity). {UniProtKB:P22366, PubMed:15361868, PubMed:18292575, PubMed:19506249, PubMed:20855887, PubMed:24316379, PubMed:33718825, PubMed:9013863}.

Additionnal Information

Immunoblotting: use at 1:500-1:1,000 dilution. <br><br>Positive control: Whole cell lysate from Jurkat cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human and mouse MyD88 (35 kD).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Cellular responses induced by the pro- inflammatory cytokine IL-1 require IL-1 receptor complex (IL-1R1 and IL- 1RacP). Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor. Dominant negative mutants of MyD88 attenuate IL-1R- mediated NF-kappaB activation. MyD88 also functions as a regulator molecule for IL- 18 receptor and human Toll receptor, members of the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to LPS which require Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory responses induced by cytokines IL-1, IL-18, and LPS. MyD88 is expressed in a variety of tissues.