Mouse anti Yeast GAL-4 Activation Domain

GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.

Antibody can be used for Western Blotting (5-10µg/mL). Recognizes GAL4 derivatives containing the C-terminal activation domain. Optimal concentration should be evaluated by serial dilutions.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

1. GAL4 is regulated by a glucose-responsive functional domain. Stone, G. , Sadowski, I. EMBO J. 1993, 12, 1375-1385._x000D_ 2. An amino-terminal fragment of GAL4 binds DNA as a dimer. Carey, M., et al. J. Mol. Biol. 1989, 209, 423-432._x000D_ 3. The DNA binding domains of the yeast Gal4 and Human c-Jun transcription factors interact through the zinc-finger and bZIP motifs. Sollerbrant, K., et al. Nucleic Acids Res. 1995, 23, 588-594.