Mouse anti Yeast GAL-4 Activation Domain

Product Specifications

Background

GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.

CAS Number

9007-83-4

Host

Mouse

Species Reactivity

Yeast

Isotype

IgG1

Clone

5C8-5

Conjugation

Unconjugated

Type

Monoclonal Antibody

Source

Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells.

Applications

WB, IP

Field of Research

Molecular Biology

Purification Method

Protein A/G Chromatography

Assay Principle

Antibody can be used for Western Blotting (5-10µg/mL). Recognizes GAL4 derivatives containing the C-terminal activation domain. Optimal concentration should be evaluated by serial dilutions.

Stability

See expiration date on vial

Concentration

See vial for Concentration

Form

Provided as solution in phosphate buffered saline with 0,08% sodium azide

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

References & Citations

1. GAL4 is regulated by a glucose-responsive functional domain. Stone, G. , Sadowski, I. EMBO J. 1993, 12, 1375-1385._x000D_ 2. An amino-terminal fragment of GAL4 binds DNA as a dimer. Carey, M., et al. J. Mol. Biol. 1989, 209, 423-432._x000D_ 3. The DNA binding domains of the yeast Gal4 and Human c-Jun transcription factors interact through the zinc-finger and bZIP motifs. Sollerbrant, K., et al. Nucleic Acids Res. 1995, 23, 588-594.