Biotinylated Human HLA-A*02:01&B2M&HCV NS3 (CINGVCWTV) Complex Protein (Monomer, HPLC verified)

Hepatitis C virus (HCV) is a single-stranded RNA virus that causes hepatitis C. It is prone to causing chronic hepatitis and can progress to liver cirrhosis and liver cancer. NS3 has serine protease activity (at the N-terminus, assisting in the cleavage of viral polyproteins) and helicase/nucleoside triphosphatase activity (at the C-terminus, participating in RNA unwinding and replication), and it is the main target of anti-HCV virus drugs (such as protease inhibitors) . The Human HLA-A*0201 HCV NS3 (CINGVCWTV) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M and CINGVCWTV peptide of the HCV NS3.

Product Specifications

Background

Specifications

Biotinylated Human HLA-A*02:01&B2M&HCV NS3 (CINGVCWTV) Complex Protein is produced by co-expression of HLA-A and B2M loaded with HCV NS3 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 40-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAA59606.1 (HLA-A*0201) & P61769-1 (B2M) & CINGVCWTV

Expression Region

Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & CINGVCWTV peptide

Host

HEK293

Target

HLA-A*0201 & B2M & HCV NS3 (CINGVCWTV)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.