Mouse Th1/Th2 Cytokine Kit (6-plex) (Flow Cytometry Multiplex Bead Assay, Non-Magbeads)

Mouse Th1/Th2 Cytokine Kit (6-plex) (Flow Cytometry Multiplex Bead Assay, Non-Magbeads) gives quantitative results of f Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-6 (IL-6), Interleukin-10 (IL10), Tumor Necrosis Factor α type (TNF-α) and Interferon-γ (IFN-γ) with a sample testing. The performance of this kit has been optimized for specific analysis of cytokines in mouse serum samples.

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Mouse Th1/Th2 Cytokine 6-plex panel is a multiplex bead-based indirect immunofluorescence assay, utilizing flow cytometry for data acquisition and classification. The beads conjugated with specific antibody, target protein (mouse IL-2/4/6/10, TNF-α, IFN-γ) in specimens and Biotin-conjugated detection antibody form a sandwiches complex as bead-analyte-detection antibody. Streptavidin-phycoerythrin (SA-PE) is subsequently added and would bind to biotinylated detection antibodies. The intensity of PE fluorescence signal in proportion to the target protein in specimens, are assessed by flow cytometry. The present assay has been optimized for quntitatively analysis for the mouse IL-2/4/6/10, TNF-α, IFN-γ in mouse serum samples.