Human IL-4 / IL-4RA Inhibitor Screening Kit (TR-FRET)

1. Unopened kit should be stored at 2°C-8°C upon receiving. 2. Find the expiration date on the outside packaging and do not use reagents past their expiration date. 3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

This Human IL-4/IL-4RA Inhibitor Screening Kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) . Use a mixture of biotinylated Human IL-4 and Europium-chelate labeled streptavidin as the Donor and FA labeled Human IL-4 RA as the Acceptor. — In the absence of inhibitors for Human IL-4 binding to Human IL-4RA, the Donor and Acceptor are in close proximity due to the binding of Human IL-4 and Human IL-4RA. Upon excitation with a specific light source, the Donor emits a 620 nm signal, which is absorbed by the Acceptor, resulting in a 665 nm emission. — In the presence of inhibitors that block the binding of Human IL-4 to Human IL-4RA, the Donor-Acceptor interaction is disrupted, preventing FRET from occurring.