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Recombinant Acetyl-Histone H3 Monoclonal Antibody

This is a Recombinant Acetyl-Histone H3 Recombinant Antibody from Reddot Biotech. This product is for Research Use Only.

Product Specifications

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Synonyms

H3 histone family, member A, H3/A, H31, H3FA, Hist1h3a, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone 1, H3a, Histone cluster 1, H3a, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3

Gene ID

8290

Swiss Prot

P68431

Reactivity

Human

Immunogen

A synthetic acetyl-peptide corresponding to residues surrounding Lys27 of human Histone H3

Clonality

Monoclonal

Conjugation

Unconjugated

Type

Recombinant Antibody

Applications

WB, IHC-P, IF

Purification Method

Affinity purification

Assay Type

Antibody

Concentration

300 μg/mL

Dilution

WB 1:500-1:1000, IHC 1:50-1:100, IF 1:50-1:100

Buffer

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

74kDa

Observed Molecular Weight

74/48kDa

Host or Source

Rabbit

Isotype

IgG

Available Sizes

Curated Selection

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