Recombinant Vimentin Monoclonal Antibody
This is a Recombinant Vimentin Recombinant Antibody from Reddot Biotech. This product is for Research Use Only.
Product Specifications
Background
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein,55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
Synonyms
CTRCT30, Epididymis luminal protein 113, FLJ36605, HEL113, VIM, VIME, Vimentin
Gene ID
7431
Swiss Prot
P08670
Reactivity
Human, Mouse, Rat, Hamster
Immunogen
A synthetic peptide of human Vimentin
Clonality
Monoclonal
Conjugation
Unconjugated
Type
Recombinant Antibody
Applications
WB, IHC-P, IF
Purification Method
Affinity purification
Assay Type
Antibody
Concentration
300 μg/mL
Dilution
WB 1:500-1:1000, IHC 1:50-1:100, IF 1:50-1:100
Buffer
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Calculated Molecular Weight
134kDa
Observed Molecular Weight
175kDa
Host or Source
Rabbit
Isotype
IgG
Available Sizes
Curated Selection
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