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His-Tag Monoclonal Antibody

This is a His-Tag Tags and Controls Antibody from Reddot Biotech. This product is for Research Use Only.

Product Specifications

Background

The 6xHis tag, also known as polyhistidine tag, His6 tag and/or hexa histidine tag, is an amino acid motif consisting of at least 6 histidine residues fused to the carboxyl (C-) or amino (N-) terminus of a target protein in transfected cells. It is one of the simplest and most widely used purification tags in the world.

Synonyms

6 His epitope tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, His tag, Polyhistidine Tag

Reactivity

All

Immunogen

Synthetic peptide corresponding to 6His tag conjugated to keyhole limpet haemocyanin.

Clonality

Monoclonal

Conjugation

Unconjugated

Type

Tags and Controls Antibody

Applications

WB, IP, FC

Purification Method

Protein A/G purification

Assay Type

Antibody

Concentration

1mg/mL

Dilution

WB 1:1000-1:2000, IP 3ug/sample, FCM:0.2 ug

Buffer

PBS with 0.1% sodium azide, 50% glycerol, pH 7.4

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

40kDa

Observed Molecular Weight

40kDa

Host or Source

Mouse

Isotype

IgG1

Available Sizes

Frequently Asked Questions

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