Anti-KLH | Keyhole limpet hemocyanin, Biotin-conjugated (40 µg)
Product Specifications
Background
Keyhole limpet hemocyanin(KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea molluskMegathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host’s immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.
Host
Rabbit
Reactivity
Megathura crenulata- most commonly used carrier protein
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Purified keyhole limpet hemocyanin (KLH), whole molecule.
Clonality
Polyclonal
Applications
ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
Dilution
1: 5 000 (ELISA), 1: 5 000 (WB), 1: 1000 (IL)
Purity
Immunogen affinity purified serum in PBS, pH 7.4, conjugated to biotin.
Format
Liquid
Molecular Weight
ca. 400 kDa/subunit
Precautions
Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies, Due to its large size KLH protein will be very difficult to separate on SDS-PAGE
Storage Conditions
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70a994c49e6dc66fb4e/datasheet/as99 001b.pdf
CAS Number
9007-83-4
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