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Anti-V5 (mouse antibody (monoclonal)

Product Specifications

Background

V5-tag is a tag that can be added to a protein of interest as a fusion protein to enable purification and detection. It is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5) . Addition of a V5-Tag to a protein of interest makes it possible to localize a specific gene product in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.

CAS Number

9007-83-4

HS Code

38221900

Host

Mouse

Reactivity

V5-tagged fusion proteins

Immunogen

KLH-conjugated GKPIPNPLLGLDST synthetic peptide

Clonality

Monoclonal

Applications

ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)

Field of Research

DIG/HA/HSV/KT3/Myc/S/T7/V5/VSV-G

Dilution

1: 50 - 1: 200 (IF), 1: 200 - 1: 2000 (IP), 1: 500 -1 : 5000 (WB)

Purity

Affinity purified in PBS pH 7.4. Contains 0.02 % sodium azide. Contains 50 % glycerol.

Format

Liquid

Molecular Weight

Depends upon MW of fused protein

References & Citations

To be added when available, this antibody was released in October 2021.

Storage Conditions

Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Isotype

IgG1a

Frequently Asked Questions

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