Anti-V5 (mouse antibody (monoclonal)
Product Specifications
Background
V5-tag is a tag that can be added to a protein of interest as a fusion protein to enable purification and detection. It is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5) . Addition of a V5-Tag to a protein of interest makes it possible to localize a specific gene product in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.
CAS Number
9007-83-4
HS Code
38221900
Host
Mouse
Reactivity
V5-tagged fusion proteins
Immunogen
KLH-conjugated GKPIPNPLLGLDST synthetic peptide
Clonality
Monoclonal
Applications
ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Field of Research
DIG/HA/HSV/KT3/Myc/S/T7/V5/VSV-G
Dilution
1: 50 - 1: 200 (IF), 1: 200 - 1: 2000 (IP), 1: 500 -1 : 5000 (WB)
Purity
Affinity purified in PBS pH 7.4. Contains 0.02 % sodium azide. Contains 50 % glycerol.
Format
Liquid
Molecular Weight
Depends upon MW of fused protein
References & Citations
To be added when available, this antibody was released in October 2021.
Storage Conditions
Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Isotype
IgG1a
Documents
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