Anti-Tubulin alpha chain, Biotin conjugated (clone TU-01)

Product Specifications

Background

Tubulin alpha (TUA)together with beta tubulin is making up microtubules.The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport.

Host

Mouse

Reactivity

Arabidopsis thaliana, Eisenia, Nicotiana tabacum, Paramecium, Saccharomyces cerevisiae,human, mouse, pig, turkey

Immunogen

Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization. UniProt:Q71U36

Clonality

Monoclonal

Applications

Immunohistochemistry (IHC) paraffin, Immunocytochemistry (ICC), Flow cytometry (Flowcyt), Western blot (WB)

Dilution

1-2 μg/mL (WB)

Purity

Conjugated with biotin-LC-NHS under optimum conditions and free of unconjugated biotin

Format

Liquid at 1 mg/mL.

Molecular Weight

50 | 54 kDa

Storage Conditions

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.