Anti-GST tag (mouse monoclonal) (clone S-tag-05)
Product Specifications
Background
GST-tag (glutathione S-transferase) from a parasite Schistosoma japonicum is a tag added to a protein of interest as a fusion protein for protein purification and detection. It allows purification by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
CAS Number
9007-83-4
HS Code
38221900
Host
Mouse
Reactivity
GST fusion proteins
Immunogen
GST protein
Clonality
Monoclonal
Applications
Immunoprecipitation (IP), Western blot (WB)
Field of Research
BC2/Biotin/BirA/DYKDDDDK/GST/Halo/His/...
Dilution
1 µg/ml (WB)
Purity
Immunoglobulin purified on Protein A.
Format
Liquid at 1 mg/ml.
Additionnal Information
This antibody is recognizing native and denatured fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems. It can be also used for immuno purification of GST-tagged proteins.
References & Citations
To be added when available, antibody released in October 2021.
Storage Conditions
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Isotype
IgG2b
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