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Anti-GST tag (mouse monoclonal) (clone S-tag-05)

Product Specifications

Background

GST-tag (glutathione S-transferase) from a parasite Schistosoma japonicum is a tag added to a protein of interest as a fusion protein for protein purification and detection. It allows purification by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.

CAS Number

9007-83-4

HS Code

38221900

Host

Mouse

Reactivity

GST fusion proteins

Immunogen

GST protein

Clonality

Monoclonal

Applications

Immunoprecipitation (IP), Western blot (WB)

Field of Research

BC2/Biotin/BirA/DYKDDDDK/GST/Halo/His/...

Dilution

1 µg/ml (WB)

Purity

Immunoglobulin purified on Protein A.

Format

Liquid at 1 mg/ml.

Additionnal Information

This antibody is recognizing native and denatured fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems. It can be also used for immuno purification of GST-tagged proteins.

References & Citations

To be added when available, antibody released in October 2021.

Storage Conditions

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Isotype

IgG2b

Frequently Asked Questions

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