Anti-ACT | Actin (recombinant monoclonal, clone 14H4G8)
Product Specifications
Background
Actinis a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
Host
Mouse
Reactivity
Arabidopsis thaliana, Medicago sativa, Zea mays
Not reactive in
Chlamydomonas reinhardtii
Immunogen
Around 100 amino acids of recombinant actin conserved more than 80% inArabidopsis thaliana: actin-1P0CJ46AT2G37620, actin-2Q96292AT3G18780, actin-3P0CJ47AT3G53750, actin-4P53494AT5G59370, actin-5Q8RYC2At2g42100, actin-7P53492At5g09810, actin-8Q96293AT1G49240, actin-11P53496AT3G12110, actin-12P53497AT3G46520
Clonality
Monoclonal
Applications
Immunofluorescence (IF), Western blot (WB)
Dilution
1: 500 (IF), 1 : 1000 1: 5000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl, of sterile or deionized water
Molecular Weight
41.6 | 45 kDa
Precautions
This antibody is compatible with a secondary antibody for fluorescence detection: goat anti-mouse secondary antibody (IRDye 800CW, Li-cor)
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc66ff64/datasheet/as21 4615.pdf
CAS Number
9000-83-3
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