Anti-ACT | Actin (recombinant monoclonal, clone 14H4G8)
Product Specifications
Background
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
CAS Number
9000-83-3
HS Code
38221900
Gene ID
818339
UniProt
P0CJ46 , Q96292 , P0CJ47 , P53494 , Q8RYC2 , P53492 , Q96293 , P53496 , P53497
Host
Mouse
Reactivity
Arabidopsis thaliana, Medicago sativa, Zea mays
Not reactive in
Chlamydomonas reinhardtii
Immunogen
Around 100 amino acids of recombinant actin conserved more than 80% in Arabidopsis thaliana: actin-1 P0CJ46 AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47 AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293 AT1G49240, actin-11 P53496 AT3G12110, actin-12 P53497 AT3G46520
Clonality
Monoclonal
Applications
Immunofluorescence (IF), Western blot (WB)
Field of Research
Cytoplasm marker
Dilution
1: 500 (IF), 1 : 1000 1: 5000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl, of sterile or deionized water
Molecular Weight
41.6 | 45 kDa
Precautions
This antibody is compatible with a secondary antibody for fluorescence detection: goat anti-mouse secondary antibody (IRDye 800CW, Li-cor)
Additionnal Information
This antibody was produced using CHO cells expression system.
References & Citations
To be added when available, antibody released in October 2021.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Agropyron cristatum, Beta vulgaris, Betula luminifera, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Castanea sativa, Chorispora bungeana, Cyanidioschyzon merolae strain 10D, Glycine max, Glycine soja, Halogeton glomeratus, Medicago truncatula, Malus domestica, Oryza sativa, Pisum sativun, Solanum lysopersicum, Solanum tuberosum, Phaseolus vulgaris, Picea abies, Picea sitchensis, Prunus avium, Ricinus communis, Rubus plicatus, Theobroma cacao, Triticum aestivum, Zea mays, Vicia fabaSpecies of your interest not listed? Contact us
Isotype
IgG1
Documents
Frequently Asked Questions
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