Anti-EGFP | Enhanced Green Fluorescence Protein
Product Specifications
Background
EGFP is an Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca (2+) -activated photoprotein aequorin. Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly- (N+2), and oxidation of Tyr- (N+1) to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
CAS Number
9007-83-4
HS Code
38221900
Host
Rabbit
Reactivity
GFP, EGFP
Not reactive in
MCherry
Immunogen
Recombinant GFP from Aequorea coerulescens (belt jellyfish) overexpressed and purified from E.coli, UniProt: Q6YGZ0
Clonality
Polyclonal
Applications
Immunocytochemistry (ICC), Western blot (WB)
Field of Research
BFP/FITC/GFP/RFP/TRITC/YFP
Dilution
1:2000 - 1:5000 (ICC), 1:1000 - 1:5000 (WB)
Purity
Immunogen affinity purified in PBS pH 7.2-7.6, 0.1% trehalose, 5 mM sodium azide.
Format
Lyophilized
Reconstitution
For reconstitution add 100 µl of sterile water, ultrapure water
Molecular Weight
27 kDa (for GFP without fusion partner)
Precautions
EGFP has Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000
References & Citations
To be added when available.
Storage Conditions
Store lypholized antibody at 4 ºC. After reconstitution keep aliquots at -20ºC for a higher stability. Avoid repetitive freeze/thaw cycles. Centrifuge briefly to remove any insoluble material.Expiry date: 12 months after purchase if unopened
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