Anti-APP-C31 | Caspase 3 cleaved APP, C terminal fragment

Product Specifications

Background

The Alzheimer Amyloid Precursor Protein (APP) is a transmembrane protein whose abnormal processing is associated with the pathogenesis of Alzheimer’s disease. APP695 lacking the protease inhibitor domain is the predominant form in neuronal tissues. APP695 is cleaved by caspases into the 664-residue amino (N) -terminal fragment that lacks the carboxyl C-terminal 31-residues (APPC31) and the 31-residues C-terminal fragment (APP-C31) . Both fragments might be potent inducers of neuronal apoptosis. An antibody (named ACT1) against the N-terminus of caspase 3-generated APP C-terminal 31 aa of human APP695 (APP-C31) was raised in rabbit.

HS Code

38221900

Gene ID

836

UniProt

P42574

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to the N-terminal of human caspase 3-generated APP C-terminal 31 amino acids (aa 665-670) UniProt: P05067

Applications

ELISA (ELISA), Immunolocalisation (IL), Western blot (WB)

Field of Research

Alzheimer's disease

Dilution

1::500 - 1: 1000 (IL), 1:1000-1:3000 (WB)

Purity

Serum. Contains 0.05% sodium azide.

Format

Liquid

Reconstitution

For reconstitution, add µl of sterile water

Molecular Weight

86.9 | 100 kDa

References & Citations

Nishimura et al. (2002) Cell death induced by a caspase-cleaved transmembrane fragment of the Alzheimer amyloid precursor protein. Cell Death Differ. 2002 Feb;9 (2) :199-208. doi: 10.1038/sj.cdd.4400931. PMID: 11840170.

Storage Conditions

Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.