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Anti-RAD51 | DNA repair protein RAD51 (Saccharomyces cerevisiae) (ChIP grade)

Selected Variant

AS21 4549

Size: 100 µg

Product Specifications

Background

S. cerevisiae Rad 51 protein (400 aa, 43 kDa) is a functional and structural homolog of E.coli RecA and human Rad51 proteins and plays a central role in DNA homologous recombination and recombination repair by promoting homologous DNA strand exchange reaction. Dmcl, Rad55, Rad57 are paralogs of Rad51 and they form complex with Rad51 and Rad52 in mediating recombination processes.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

856831

UniProt

P25454

Host

Rabbit

Reactivity

Saccharomyces cerevisiae

Immunogen

Purified, full length, recombinant and HIS tagged RAD51 protein from Saccharomyces cerevisiae, UniProt: P25454

Clonality

Polyclonal

Applications

ELISA (ELISA), Chromatin immunoprecipitation (ChIP), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)

Dilution

1: 500 : 2000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4. Contains 50 % glycerol, filter sterilized.

Format

Liquid

Molecular Weight

43 kDa

Additionnal Information

ChIP application is described in Ribeyre and Shore 2012 while immunofluorescence is described in Muramoto et al 218.

References & Citations

Muramoto et al. (2018) Phenotypic diversification by enhanced genome restructuring after induction of multiple DNA double-strand breaks. Nat Commun. 2018 May 18;9 (1) :1995. doi: 10.1038/s41467-018-04256-y. PMID: 29777105; PMCID: PMC5959919. (IF application) Ribeyre & Shore (2012) . Anticheckpoint pathways at telomeres in yeast. Nat Struct Mol Biol. 2012 Feb 12;19 (3) :307-13. doi: 10.1038/nsmb.2225. PMID: 22343724. (ChIP application)

Storage Conditions

Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Galdieria sulphuraria (red algae) Species of your interest not listed? Contact us

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