RuvC (protein, positive control)

Product Specifications

Background

E. coliRuvCprotein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction cleaving and resolving the recombinant.

Applications

Western blot (WB)

Purity

Contans 50% glycerol, 10 mM Tris-HCl (pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol. Over 95% pure by SDS-PAGE.

Format

Liquid

Molecular Weight

18.7 | 19 kDa

Precautions

RuvC protein is full length, highly purified (over 95%, SDS-PAGE), recombinant without a tag at a concentration of 1 mg/mL (determined by BCA method). UniProt:P0A814

References & Citations

Shinagawa& Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584.Iwasakiet al. (1991) Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure. EMBO J. 1991 Dec;10(13):4381-9. PMID: 1661673; PMCID: PMC453191.

Storage Conditions

Store at -20°C or -80°C for a longer period of time; once make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.