RuvC (protein, positive control)

E. coli RuvC protein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction cleaving and resolving the recombinant.

RuvC protein is full length, highly purified (over 95%, SDS-PAGE), recombinant without a tag at a concentration of 1 mg/mL (determined by BCA method).  UniProt:P0A814

This product can be used in:In vitro functional studies. RuvC cleaves recombination intermediate at Holiday JunctionAs a positive control in Western blot and standard in ELISA.

Shinagawa & Iwasaki (1996) . Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21 (3) :107-11. PMID: 8882584.Iwasaki et al. (1991) Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure. EMBO J. 1991 Dec;10 (13) :4381-9. PMID: 1661673; PMCID: PMC453191.

Store at -20°C or -80°C for a longer period of time; once make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.