Anti-RuvC | Holliday junction nuclease RuvC

E. coliRuvCprotein is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces nicks at the symmetrical points of the Holliday junction, cleaving and resolving the recombinant.

Ichiyanagiet al. (1998). Mutational analysis on structure-function relationship of a holliday junction specific endonuclease RuvC. Genes Cells. 1998 Sep;3(9):575-86. doi: 10.1046/j.1365-2443.1998.00213.x. PMID: 9813108.Shinagawa& Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584.Saitoet al (1995). Identification of four acidic amino acids that constitute the catalytic center of the RuvC Holliday junction resolvase. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7470-4. doi: 10.1073/pnas.92.16.7470. PMID: 7638215; PMCID: PMC41361.

Store at 4°C for 6 monthss, afterwards at -80°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.