Anti-LexA | LexA repressor

Product Specifications

Background

LexA represor binds specifically to the SOS-box sequence and represses the genes belonging to the SOS regulation. In response to DNA damage, RecA protein is activated by ss-DNA accumulated in the damaged cells and promotes autocleavage of LexA repressor by its coprotease activity. As a result, DNA repair genes and error prone polymerases are induced, and DNA damage is repaired and mutation is induced (1) . The lexA gene is used for yeast two-hybrid experiments as a bait to identify the protein-protein interaction in vivo.Alternative names: exrA, spr, tsl, umuA

CAS Number

9007-83-4

HS Code

38221900

Gene ID

948544

UniProt

P0A7C2

Host

Rabbit

Reactivity

Escherichia coli

Immunogen

Full-size highly-purified recombinant LexA protein, UniProt: P0A7C2

Clonality

Polyclonal

Applications

Chromatin immunoprecipitation (ChIP), Immunolocalisation (IL), Western blot (WB)

Field of Research

Bacteria

Dilution

1: 1000 - 1: 3000 (WB)

Purity

Serum. Contains 0.05% sodium azide.

Format

Liquid

Molecular Weight

22.3 | 23 kDa

Additionnal Information

This product can be used in:Studies of SOS regulation in E.coliDetection of bait constructs in yeast two-hybrid systemImmunolocalization of LexA fusion proteins (fixed with 4 % formaldehyde) Immunoprecipiation and ChIP

References & Citations

Hishida et al (2004) Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev. 2004 Aug 1;18 (15) :1886-97. doi: 10.1101/gad.1223804. PMID: 15289460; PMCID: PMC517408.

Storage Conditions

Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.