What is the catalog number for Anti-mNeonGreen | Fluorescent Protein?

The catalog number for Anti-mNeonGreen | Fluorescent Protein is AS21 4525.

What sizes are available for Anti-mNeonGreen | Fluorescent Protein?

Anti-mNeonGreen | Fluorescent Protein is available in the following size(s): 50 µg.

Is Anti-mNeonGreen | Fluorescent Protein in stock?

Anti-mNeonGreen | Fluorescent Protein is currently made to order / not held in immediate stock. Contact us for lead time.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

Anti-mNeonGreen | Fluorescent Protein

Product Specifications

Background

MNeonGreen (Fluorescent Protein) is a new bright monomertic yellow-green fluorescent, with low conservation level to GFP. It has an excitation maximum at 506 nm and an emission maximum at 517 nm and therefore is compatible with the most GFP filter sets. mNeonGreen is 3x brighter compare to GFP, more stable and does not bleach so fast as GFP, which makes it very suitable for confocal and super resolution microscopy, for fusion proteins with low expression levels. It can be used in bicistronic vectors, which will allow simultaneous expression of two proteins separately, from the same RNA transcript. mNeonGreen has MW of 26.6 kDa.

CAS Number

9007-83-4

HS Code

38221900

UniProt

A0A1S4NYF2

Host

Rabbit

Reactivity

MNG tag in plant (Arabidopsis thaliana) and algal vectors (Chlamydomonas reinhardtii)

Not reactive in

No confirmed exceptions from predicted reactivity are currently known.

Immunogen

KLH-conjugated peptide derived from synthetic peptide, UniProt: A0A1S4NYF2 from common lancelet Branchiostoma lanceolatum

Clonality

Polyclonal

Applications

Immunofluorescence (IF), Western blot (WB)

Field of Research

BFP/FITC/GFP/RFP/TRITC/YFP

Dilution

1 µg/1ml (IF), 1 : 1000 - 1: 5000 (WB)

Purity

Immunogen affinity purified serum, in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water.

Molecular Weight

Depends upon used fusion partner

Precautions

This antibody is detecting protein sequence of mNG tag in plant and algal vectors.This antibody is also reacting to some degree with YFP and mCherry.

Additionnal Information

The peptide used to elicit this antibody is conserved in the following expression vectors: dCas9-P2A-DHFR (TSc3) -T2A-mNeonGreen [Cloning vector pBM020]Cas9-P2A-DHFR (TSc3) -T2A-mNeonGreen [Cloning vector pBM006]mNeonGreen4-tDeg [Cloning vector pminiCMV-mNeonGreen4-tDeg]ER-localized mNEONGREEN [Binary vector pKT-NM-erNEON]mNeonGreen-3xFLAG [Cloning vector pLM160-mNeonGreen]mNeonGreen-ty1 [Cloning vector pSAG1-mNeonGreen_TUB1-dTomato]mNeonGreen, partial [Binary vector pRATIO2131]The peptide used to elicit this antibody is not conserved in GFP protein sequence. Antibody is also recognzing mCheery sequence.

References & Citations

To be added when available, antibody available in November 2021.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.