Anti-GFP | Green Fluorescence Protein, clone 1A5 (rat monoclonal)
Product Specifications
Background
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
CAS Number
9007-83-4
HS Code
38221900
UniProt
P42212
Host
Rat
Reactivity
Native GFP, Recombinant GFP (E. coli), all variants of GFP, including EGFP
Immunogen
Recombinant GFP protein from Aequorea victoria, UniProt: P42212
Clonality
Monoclonal
Applications
Chromatin immunoprecipitation (ChIP), ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Field of Research
BFP/FITC/GFP/RFP/TRITC/YFP
Dilution
1 : 5000-1 : 25 000 (ELISA), 1 : 500 (IF), 1 : 2000-1 : 10 000 (WB)
Purity
Immunoglobulin Protein A purified in PBS, 50 % glycerol, filter sterilized.
Format
Liquid, 1 mg/ml.
Additionnal Information
Antibody detects GFP and EGFP
References & Citations
Maehara et al. (2015) . issue-specific expression of histone H3 variants diversified after species separation. Epigenetics Chromatin. 2015 Sep 17;8:35. doi: 10.1186/s13072-015-0027-3. (ChIP) Okazaki et al. (2012) . Nuclear localization signal in a cancer-related transcriptional regulator protein NAC1. Carcinogenesis. 2012 Oct;33 (10) :1854-62.doi: 10.1093/carcin/bgs193. (Immunoprecipiation)
Storage Conditions
Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Isotype
IgG1
Documents
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