Anti-BFP, GFP, YFP | Fluorescent Protein, clone 3A6

Product Specifications

Background

Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.

Host

Mouse

Reactivity

BFP, GFP, YFP

Immunogen

Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed inE.coli.

Clonality

Monoclonal

Applications

ELISA (ELISA), Western blot (WB)

Dilution

1 : 150-1: 3000 (ELISA), 1 : 500-1 : 5000 (WB)

Purity

Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.

Format

Lyophilized

Reconstitution

Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%).Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 •Additional sodium azide ( up to 0.05%) is recommended for long term storage. •For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.

Storage Conditions

Store lyophilized/reconstituted at 2-8°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.