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Anti-Tubulin alpha chain (monoclonal antibody)

Product Specifications

Background

Tubulin alpha (TUA) together with beta tubulin is making up microtubules.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

7846

Host

Mouse

Reactivity

Arabidopsis thaliana, Chlorella vulgaris, Eisenia, human, mouse, Nicotiana tabacum, Paramecium sp. , pig, turkey, yeast

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization, UniProt: Q71U36

Clonality

Monoclonal

Applications

Flow cytometry (FlowCyt), Immunocytochemistry (ICC), Immunohistochemistry on paraffin sections (IHC), Immunoprecipitation (IP), Western blot (WB)

Field of Research

Cytoskeleton

Dilution

1 : 500 (ICC), 1-4 µg/ml /FlowCyt), 1-4 µg/ml (WB)

Purity

Immunoglobulin Protein A purified in PBS. Contain 15 mM sodium azide.

Format

Liquid

Molecular Weight

51 kDa

Precautions

This antibody is recognizing defined epitope (amino acid 65-97) on N-terminal structural domain of alpha tubulin.Recommended secondary antibody, goat anti-mouse IgG1, HRP conjugatedAS16 3715

Additionnal Information

Metal induced stress affected the expression of tubulin, and that therefore, this protein cannot be used as a loading control under that type of conditions, data in application example

References & Citations

Liu et al. (2022) Identification of positive and negative regulators of antiviral RNA interference in Arabidopsis thaliana. Nat Commun. 2022 May 30;13 (1) :2994. doi: 10.1038/s41467-022-30771-0. PMID: 35637208; PMCID: PMC9151786.

Storage Conditions

Store at 4°C; Do not freeze. Do not exceed exipry date is provided on the tube. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Plants Species of your interest not listed? Contact us

Isotype

IgG1

Frequently Asked Questions

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