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Anti-Tubulin alpha chain (monoclonal antibody)

Product Specifications

Background

Tubulin alpha (TUA)together with beta tubulin is making up microtubules.

Host

Mouse

Reactivity

Arabidopsis thaliana, Chlorella vulgaris, Eisenia, human, mouse,Nicotiana tabacum,Parameciumsp. , pig, turkey, yeast

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization, UniProt:Q71U36

Clonality

Monoclonal

Applications

Flow cytometry (FlowCyt), Immunocytochemistry (ICC), Immunohistochemistry on paraffin sections (IHC), Immunoprecipitation (IP), Western blot (WB)

Dilution

1 : 500 (ICC), 1-4 µg/mL /FlowCyt), 1-4 µg/mL (WB)

Purity

Immunoglobulin Protein A purified in PBS. Contain 15 mM sodium azide.

Format

Liquid

Molecular Weight

51 kDa

Precautions

This antibody is recognizing defined epitope (amino acid 65-97) on N-terminal structural domain of alpha tubulin.Recommended secondary antibody, goat anti-mouse IgG1, HRP conjugatedAS16 3715

References & Citations

Liuet al. (2022) Identification of positive and negative regulators of antiviral RNA interference in Arabidopsis thaliana. Nat Commun. 2022 May 30;13(1):2994. doi: 10.1038/s41467-022-30771-0. PMID: 35637208; PMCID: PMC9151786.

Storage Conditions

Store at 4°C; Do not freeze. Do not exceed exipry date is provided on the tube. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Product Datasheet

https://cdn.gentaur.com/products/451/6867f70b994c49e6dc6704d3/datasheet/as20 4483.pdf

CAS Number

9007-83-4

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