Anti-GFP | Green Fluorescence Protein

Product Specifications

Background

GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.

CAS Number

9007-83-4

HS Code

38221900

UniProt

Host

Rabbit

Reactivity

Recombinant GFP overexpressed in E.coli from Arabidopsis thaliana, Chlamydomonas reinhardii, Nicotiana tabacum

Immunogen

Recombinant GFP protein derived from Aequorea victoria, UniProt: P42212

Clonality

Polyclonal

Applications

Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western blot (WB)

Field of Research

BFP/FITC/GFP/RFP/TRITC/YFP

Dilution

1: 5000 - 1 : 10 000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

References & Citations

Dieren et al. (2024) . Analysis of abiotic and biotic stress-induced Ca2+ transients in the crop species Solanum tuberosum. Sci Rep . 2024 Nov 11;14 (1) :27625. doi: 10.1038/s41598-024-79134-3.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.