Anti-His-tag | 3xHis (polyclonal)
Product Specifications
Background
His-Tag is a polyhistidine tag which consists of 3 or 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
CAS Number
9007-83-4
HS Code
38221900
UniProt
UniProt
Host
Rabbit
Reactivity
3xHis tagged proteins
Immunogen
KLH-conjugated synthetic peptide 3xHis
Clonality
Polyclonal
Applications
Immunofluorescence (IF), Western blot (WB)
Field of Research
BC2/Biotin/BirA/DYKDDDDK/GST/Halo/His/...
Dilution
1 µg/mL (IF), 1: 1000 - 1 : 5000 (WB)
Purity
Antigen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl, of sterile water
Molecular Weight
3xHis tagged proteins
References & Citations
To be added when available, antibody available in June 2021.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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