Anti-BirA (mutated/TurboID) - ALP conjugated (40 µg)
Selected Variant
AS20 4440-ALP
Size: 40 µg
Product Specifications
Background
Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019) . Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. This tag has MW of 35 kDa.
CAS Number
9007-83-4
HS Code
38221900
UniProt
UniProt
Host
Rabbit
Reactivity
BirA (mutated/TurboID)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177) . Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag) .
Clonality
Polyclonal
Field of Research
BC2/Biotin/BirA/DYKDDDDK/GST/Halo/His/...
Dilution
1 : 5000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.
Format
Liquid
Molecular Weight
Depends upon fustion partner
Storage Conditions
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
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