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Anti-BirA (mutated/TurboID) - ALP conjugated (40 µg)

Selected Variant

AS20 4440-ALP

Size: 40 µg

Product Specifications

Background

Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019) . Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. This tag has MW of 35 kDa.

CAS Number

9007-83-4

HS Code

38221900

UniProt

UniProt

Host

Rabbit

Reactivity

BirA (mutated/TurboID)

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177) . Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag) .

Clonality

Polyclonal

Field of Research

BC2/Biotin/BirA/DYKDDDDK/GST/Halo/His/...

Dilution

1 : 5000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.

Format

Liquid

Molecular Weight

Depends upon fustion partner

Storage Conditions

Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

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