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Anti-Glutamine synthetase (leaf, root)

Product Specifications

Background

Glutamine synthetase plays a role in the flow of nitrogen into nitrogenous organic compounds. There are five root types of GS isoproteis (GS1-1~ GS1-5) and one chloroplastic GS isoprotein (GS2/GLN2) are known. The sequence identity between GS1-1 and GS2 is 65%.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

542520

UniProt

P38559

Host

Rabbit

Reactivity

Arabidopsis thaliana, Spinacia oleracea, Zea mays

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Purified full length, tag cleaved, recombinant Zea mays GS-1 (Glutamine synthetase, root isozyme 1) UniProt: P38559

Clonality

Polyclonal

Applications

ELISA (ELISA), Western blot (WB)

Field of Research

Nitrogen Metabolism

Dilution

Assay dependent (ELISA), 1: 1000 - 1: 5000 (WB)

Purity

Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.

Format

Liquid at 2 mg/ml.

Molecular Weight

39 kDa | 43 kDa (chloroplast isoform)

Precautions

No confirmed exceptions from predicted reactivity are currently known

Additionnal Information

This antibody reactis with all glutamine synthetase isotypes from leaf and root

References & Citations

Kimata-Ariga and Hase (2014) . Multiple complexes of nitrogen assimilatory enzymes in spinach chloroplasts: possible mechanisms for the regulation of enzyme function. PLoS One . 2014 Oct 1;9 (10) :e108965. doi: 10.1371/journal.pone.0108965. Sakaibara et al. (1996) . Molecular identification and characterization of cytosolic isoforms of glutamine synthetase in maize roots. J Biol Chem. 1996 Nov 22;271 (47) :29561-8. doi: 10.1074/jbc.271.47.29561.

Storage Conditions

Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Brachypodium distachyon, Cucurbita moschata, Glycine max, Medicago truncatula, Oryza sativa, Panicum hallii, Pontederia crassipes, Populus tremula x Populus alba, Prunus persica, Raphanus sativus, Saccharum officinarum, Setaria italica, Sorghum bicolor, Triticum aestivumSpecies of your interest not listed? Contact us

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