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Anti-NAI2 | TSA1-like protein (ER lumen marker)

Selected Variant

AS20 4421

Size: 200 µg

Product Specifications

Background

NAI2 TSA1-like protein is responsible for the ER body formation. Regulates the number and shape of the ER bodies and the accumulation of PYK10 in ER bodies, but is not involved in the expression of PYK10. Interacts directly or indirectly with MEB1 and MEB2. Expressed in roots. Detected in shoot apex. Induced by NAI1. The protein is localising to ER lumen.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

820839

UniProt

Q9LSB4

Host

Rabbit

Reactivity

Arabidopsis thaliana

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Purified recombinant NAI2 of Arabidopsis thaliana, residues 272-502 with a His tag, UniProt: Q9LSB4, TAIR: At3g15950

Clonality

Polyclonal

Applications

Immunofluorescence (IF), Western blot (WB)

Field of Research

ER marker

Dilution

1: 1000 - 1: 3000 (IF), 1: 2000 - 1: 4000 (WB)

Purity

Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.

Format

Liquid at 2 mg/ml.

Molecular Weight

85 | 120 kDa

Precautions

Signal peptide of 24 amino acids is removed from the mature protein

References & Citations

Ueda et al. (2018) .Endoplasmic Reticulum (ER) Membrane Proteins (LUNAPARKs) are Required for Proper Configuration of the Cortical ER Network in Plant Cells. Plant Cell Physiol. 2018 Oct 1;59 (10) :1931-1941. doi: 10.1093/pcp/pcy137. Yamada et al. (2013) . Identification of two novel endoplasmic reticulum body-specific integral membrane proteins. Plant Physiol. 2013 Jan;161 (1) :108-20. doi: 10.1104/pp.112.207654. Yamada et al. (2008) . NAI2 is an endoplasmic reticulum body component that enables ER body formation in Arabidopsis thaliana. Plant Cell . 2008 Sep;20 (9) :2529-40. doi: 10.1105/tpc.108.059345.

Storage Conditions

Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

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