Anti-Beta-tubulin, (loading control antibody), mouse monoclonal
Product Specifications
Background
Beta-tubulin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism. Tubulin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot.
CAS Number
9007-83-4
HS Code
38221900
Host
Mouse
Reactivity
Chicken, human, monkey, mouse, rat, rabbit
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Synthetic peptide derived from the N-terminal of the human beta-tubulin
Clonality
Monoclonal
Applications
Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Field of Research
Human Developmental Biology
Dilution
1: 2000 - 1: 5000 (WB), for other techniques end user needs to determine most optimal diltion
Purity
IgG2a purified on Protein A n 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
Format
Lyophilized
Molecular Weight
50 kDa
Precautions
This antibody recognizes both the native and denatured forms of Beta-tubulin
Additionnal Information
10mM PBS, pH 7,2, with 1% BSA and 0,05% sodium azide
Storage Conditions
Store at -20°C for 3 years or more, Reconstitute with distilled water to desired concentration before use, Aliquot to avoid repeated freeze-thaw cycles, Store aliquots at 4°C for several days to weeks
Isotype
IgG2a
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