Anti-CAH6 | Carbonic anhydrase 6
Product Specifications
Background
CAH6 (Carbonic anhydrase 6)is a zinc-containing metalloenzymes that catalyze the reversible hydration of CO2. There are three evolutionary not related families of carbonic anhydrases: alpha, beta and gamma. CAH6 belonds to the beta family and initially was localized to chloroplastsMitraet al. (2004).However, lately localization to flagella has been confirmedMackinderet al. 2017.
Host
Rabbit
Reactivity
Chlamydomonas reinhardtii
Not reactive in
Lycopersicum esculentum, Neopyropia yezoensis(macroalga),Zea mays
Immunogen
Recombinant, full length CAH6 ofChlamydomonas reinhardtii, excised from the gel, UniProt:Q6S7R9
Clonality
Polyclonal
Applications
Western blot (WB)
Dilution
1 : 1000 (WB)
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl, of sterile water
Molecular Weight
28 kDa
Precautions
Lately localization to flagella has been confirmed for CAH6Mackinderet al. 2017.Extraction method – harvest the cells and add SDS sample buffer.
References & Citations
Mackinderet al. 2017. A Spatial Interactome Reveals the Protein Organization of the Algal CO2-Concentrating Mechanism. Cell. 2017 Sep 21;171(1):133-147.e14. doi: 10.1016/j.cell.2017.08.044.Mitraet al. (2004). Identification of a new chloroplast carbonic anhydrase in Chlamydomonas reinhardtii. Plant Physiol. 2004 May;135(1):173-82.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc6700e2/datasheet/as19 4322.pdf
CAS Number
9007-83-4
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