Anti-CAH6 | Carbonic anhydrase 6

Product Specifications

Background

CAH6 (Carbonic anhydrase 6) is a zinc-containing metalloenzymes that catalyze the reversible hydration of CO2. There are three evolutionary not related families of carbonic anhydrases: alpha, beta and gamma. CAH6 belonds to the beta family and initially was localized to chloroplasts Mitra et al. (2004) .However, lately localization to flagella has been confirmed Mackinder et al. 2017.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

5728738

UniProt

Q6S7R9

Host

Rabbit

Reactivity

Chlamydomonas reinhardtii

Not reactive in

Lycopersicum esculentum, Neopyropia yezoensis (macroalga), Zea mays

Immunogen

Recombinant, full length CAH6 of Chlamydomonas reinhardtii, excised from the gel, UniProt: Q6S7R9

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

RUBISCO/Carbon metabolism

Dilution

1 : 1000 (WB)

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water

Molecular Weight

28 kDa

Precautions

Lately localization to flagella has been confirmed for CAH6Mackinderet al. 2017.Extraction method – harvest the cells and add SDS sample buffer.

References & Citations

Mackinder et al. 2017. A Spatial Interactome Reveals the Protein Organization of the Algal CO2-Concentrating Mechanism. Cell. 2017 Sep 21;171 (1) :133-147.e14. doi: 10.1016/j.cell.2017.08.044.Mitra et al. (2004) . Identification of a new chloroplast carbonic anhydrase in Chlamydomonas reinhardtii. Plant Physiol. 2004 May;135 (1) :173-82.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.