Anti-BetaCA1 | Beta carbonic anhydrase 1 (chloroplastic)

Product Specifications

Background

BetaCA1 (Beta carbonic anhydrase 1) (chloroplastic) - is a zinc metalloenzyme that interconvert CO2 and HCO3 (-) . Alternative names: ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3, ATBCA1, ATSABP3, BETA CARBONIC ANHYDRASE 1, CA1, CARBONIC ANHYDRASE 1, SABP3, SALICYLIC ACID-BINDING PROTEIN 3.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

821134

UniProt

P27140-1

Host

Rabbit

Reactivity

Arabidopsis thaliana

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated synthetic peptide, derived in the part C-terminus of BetaCA1 of Arabidopsis thaliana, UniProt: P27140, TAIR: At3g01500

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

RUBISCO/Carbon metabolism

Dilution

1 : 20 000 (WB)

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water

Molecular Weight

37.5 | 25.3 kDa

Precautions

Extraction method– Grind 50 mg of leaf tissue in a sterile microcentrifuge tube using a sterile plastic pestle. Add 132μL of Protein Extraction Buffer (1x TE, 1.2 %SDS, 2.7% sucrose, 7.5 μg mL-1 bromophenol blue) to the ground leaf tissue. Vortex the sample and keep on ice for 15 mins. Centrifuge at 14,000 rpm for five minutes using a benchtop centrifuge. Collect the supernatant and in a new sterile 0.5 ml microcentrifuge tube and discard the pellet.This antibody does not recognize betaCA2.

References & Citations

DiMario et al. (2016) . The Cytoplasmic Carbonic Anhydrases βCA2 and βCA4 Are Required for Optimal Plant Growth at Low CO2. Plant Physiol. 2016 May;171 (1) :280-93. doi: 10.1104/pp.15.01990.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.