Anti-SAE1a | SUMO-activating enzyme subunit 1A

Product Specifications

Background

SAE1a (SUMO-activating enzyme subunit 1A) is the dimeric enzyme acts as an E1 ligase for SUMO1 and SUMO2. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. Functionally redundant with its paralog SAE1B. Alternative names: SUMO-activating enzyme subunit 1-1, Ubiquitin-like 1-activating enzyme E1A.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

828596

UniProt

Q8VY78-1

Host

Rabbit

Reactivity

Arabidopsis thaliana, Zea mays

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Recombinant, full-length SAE1a of Arabidopsis thaliana, UniProt:Q8VY78-1, TAIR: At4g24940, overexpressed in E.coli

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

Autophagy-related and Ubiquitin-like Proteins

Dilution

1 : 3000 (WB)

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water

Molecular Weight

36.1 | 36 kDa

References & Citations

Saracco et al. (2007) . Genetic analysis of SUMOylation in Arabidopsis: conjugation of SUMO1 and SUMO2 to nuclear proteins is essential. Plant Physiol. 2007 Sep;145 (1) :119-34.

Storage Conditions

Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.