Anti-RPN12a | 26S proteasome regulatory subunit RPN12a

Certain proportion of both wild-type transcript and protein is still produced in therpn12a -1mutant, see Figures 2B and 2CSmalleet al. (2002), PVDF membrane instead of nitrocellulose, and use the primary antibody at a 1:3000 dilution in 1% non-fat dry milk in PBS, performing just a 1 hour incubation at room temperature, so those adjustments to the protocol may also help reduce the background and increase signal intensity.Recommended Western blot conditions:protein load: 10-20 µg/well, membrane: PVDF membrane, blocking with 10% nonfat milk 1h/RT, antibody incubation buffer: PBS-T. Primary antibody dilution: 1: 3000 in PBS with non-fat dry milk incubation RT/1h, optimisation based on obtained result regarding signal/noise ratio.

Pang et al. (2025) . The adaptor protein AP-3β disassembles heat-induced stress granules via 19S regulatory particle in Arabidopsis. Nat Commun. 2025 Feb 27;16 (1) :2039. doi: 10.1038/s41467-025-57306-7. Smalle et al. (2002) . Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.

Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.